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04.365 - TRV VIGS Improvements

Patent Reference:
US Patent No. US 7,229,829.


Dinesh-Kumar
Yale University


The TRV VIGS technology (PBL Tech ID 99.192) is now complemented by new, modified vectors and modifications building on the strength of the TRV VIGS system for studying gene function in plants. The modified TRV vectors and methods deliver the following additional benefits to the existing PBL TRV technology, more efficient silencing (including broader demonstration in more plant species), more efficient production of viral RNA and a multiple cloning site for easy insertion of sequences.


References:

Burch-Smith TM et al (2004).  Applications and advantages of virus induced gene silencing (VIGS) for gene function studies in plants.  Plant Journal 39:734-746.

Liu Y et al (2002).  Virus-induced gene silencing in tomato.  Plant Journal 31(6): 777-786.

Liu Y et al (2002).  Tobacco rar1, eds1 and npr1/nim1 like genes are required for N-mediated resistance to tobacco mosaic virus.  Plant Journal 30: 415-429.
  

Contact: Dr Lars von Borcke

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